Mouse Model of Enteropathogenic Escherichia coli Infection

• DOI: 10.1128/iai.73.2.1161-1170.2005
• Publication Date: 2005-01-21T02:03:07Z
• Authors: Suzana D. Savkovic; Jennilee Villanueva; Jerrold R. Turner; Kristina A. Matko...; Gail Hecht
• Keywords: Enteropathogenic Escherichia coli; Lamina propria; Intraepithelial lymphocyte; Intestinal mucosa; Citrobacter rodentium

ABSTRACT Enteropathogenic Escherichia coli (EPEC) is an important cause of diarrhea in humans. EPEC infection cultured intestinal epithelial cells induces attaching and effacing (A/E) lesions, alters ion transport, increases paracellular permeability, stimulates inflammation. The lack a small-animal model has restricted vivo studies examining EPEC-host interactions. aim this study was to characterize the C57BL/6J mouse as infection. We have shown that can adhere colonize epithelium mice. Animal weight water intake were not altered during 10 days proximal colon infected mice contained semisolid stool, with stool pellets forming only distal colon. In contrast, entire control formed stool. Microvillous effacement actin rearrangement, characteristic A/E seen intestine but Histological assessment revealed increased numbers lamina propria neutrophils occasional crypt abscesses, intraepithelial lymphocytes, goblet EPEC-infected Altogether, these data suggest susceptible by will provide suitable for studying consequences