Pyrophosphatase of the RoundwormAscaris suumPlays an Essential Role in the Worm's Molting and Development

• DOI: 10.1128/iai.73.4.1995-2004.2005
• Publication Date: 2005-03-22T03:57:31Z
• Authors: M. Khyrul Islam; Takeharu Miyoshi; Manabu Yamada; Naotoshi Tsuji
• Keywords: Ascaris suum; Ascaris

Previous studies indicated that inorganic pyrophosphatase of Ascaris suum (AsPPase) plays an important role in larval survival the host. Here we describe a precise for AsPPase molting and development also potential recombinant (rAsPPase) protective immunity to A. infection. Using reverse transcriptase PCR analysis, found disruption gene function by RNA interference resulted suppression mRNA levels. caused inhibition third-stage larvae (31%) native protein expression, as demonstrated 56% reduction enzyme activity quantified immunoblot immunofluorescence analyses, suggesting has process. The anatomic location hypodermis along with its elevated expression prior during process supports such role. Anti-rAsPPase immunoglobulin G (IgG) 57% lung-stage fourth-stage vitro developmental arrest. Antigenic epitopes overlapped active sites. Mice immunized rAsPPase exhibited high antigen-specific IgG antibody responses were protected (>70%) against challenge migratory-phase Splenic T cells from rAsPPase-immunized mice produced low levels helper 1-type cytokines (gamma interferon interleukin-2) but interleukin-10 response. A significantly level IgG1 subclass antibodies was mice. Our results establish critical roundworms suggest use candidate vaccine ascariasis.