Direct RNA Nanopore Sequencing of Pseudomonas aeruginosa Clone C Transcriptomes

• DOI: 10.1128/jb.00418-21
• Publication Date: 2021-11-15T18:03:42Z
• Authors: Marie-Madlen Pust; Colin Francis Dav...; Lutz Wiehlmann; Burkhard Tümmler
• Keywords: Transcription

Genome-wide gene expression of bacteria is commonly studied by high-throughput sequencing size-selected cDNA fragment libraries reverse-transcribed RNA preparations. However, the depletion rRNAs, enzymatic reverse transcription, and fragmentation, size selection, amplification during library preparation lead to inevitable losses information about initial composition pool.