Effects of H 2 and Formate on Growth Yield and Regulation of Methanogenesis in Methanococcus maripaludis

• DOI: 10.1128/jb.02141-12
• Publication Date: 2013-01-19T05:49:59Z
• Authors: Kyle C. Costa; Sung Ho Yoon; Min Pan; June A. Burn; Nitin S. Baliga; John A. Leigh
• Keywords: Formate dehydrogenase; Methanococcus; Hydrogenase; Methanobacterium

ABSTRACT Hydrogenotrophic methanogenic Archaea are defined by an H 2 requirement for growth. Despite this requirement, many hydrogenotrophs also capable of growth with formate as electron donor methanogenesis. While certain responses these organisms to hydrogen availability have been characterized, starvation not reported. Here we report that during continuous culture Methanococcus maripaludis under nutrient conditions, yields relative methane production decreased markedly either excess or excess. Analysis the several mutants suggests phenomenon occurs independently storage intracellular carbon a transcriptional response Using microarray analysis, found expression genes encoding coenzyme F 420 -dependent steps methanogenesis, including one two dehydrogenases, increased but occurred at high levels regardless limitation One gene, methylene-tetrahydromethanopterin dehydrogenase, in limitation. Expression second molybdenum-dependent formylmethanofuran and molybdenum transport specifically Of only first could support on batch where was