Nitrogen source regulates glutamine synthetase mRNA levels in Neurospora crassa

• DOI: 10.1128/jb.136.3.880-885.1978
• Publication Date: 2020-01-03T16:27:38Z
• Authors: F Sánchez; M Campomanes; C Quinto; W Hansberg; J Mora; R Palacios
• Keywords: Crassa; Glutamate synthase; Neurospora

Neurospora crassa glutamine synthetase mRNA was measured by its capacity to direct the synthesis of specific protein in a cell-free system derived from rabbit reticulocytes. N. cultures grown on glutamate as sole nitrogen source had higher activities than did those glutamine. The differences were about 10-fold when polysomal RNA used for translation and 5-fold either total cellular or polyadenylic acid-enriched used. These data indicate that exponentially growing crassa, regulates adjusting levels.