Nitrogen source regulates glutamine synthetase mRNA levels in Neurospora crassa

• DOI: 10.1128/jb.136.3.880-885.1978
• Publication Date: 2020-01-03T16:27:38Z
• Authors: F Sánchez; M Campomanes; C Quinto; W Hansberg; J Mora; R Palacios
• Keywords: Neurospora; 0303 health sciences; 03 medical and health sciences; Cell-Free System; Glutamates; Neurospora crassa; Glutamate-Ammonia Ligase; Glutamine; RNA; RNA, Messenger; Poly A

Neurospora crassa glutamine synthetase mRNA was measured by its capacity to direct the synthesis of the specific protein in a cell-free system derived from rabbit reticulocytes. N. crassa cultures grown on glutamate as the sole nitrogen source had higher mRNA activities than did those grown on glutamine. The differences were about 10-fold when polysomal RNA was used for translation and about 5-fold when either total cellular RNA or polyadenylic acid-enriched cellular RNA was used. These data indicate that in exponentially growing N. crassa, the nitrogen source regulates glutamine synthetase by adjusting specific mRNA levels.