Recombination analysis of naturally diploid Candida albicans

• DOI: 10.1128/jb.152.3.969-975.1982
• Publication Date: 2021-06-02T22:33:11Z
• Authors: R Poulter; V Hanrahan; K Jeffery; D Markie; M G Shepherd; P A Sullivan
• Keywords: Auxotrophy; Protoplast; Heterokaryon

A multiply auxotrophic strain, hOG45, was derived from Candida albicans ATCC 10261. Prototrophic revertants of this multiple auxotroph were selected after mutagenesis. These prototrophic distinguishable the original prototroph, 10261, because their mitotic instability. They gave rise to derivatives which displayed one or more requirements characteristic hOG45. Two requirements, those for adenine and methionine, frequently reappeared together in revertants. This apparent linkage ade met confirmed by protoplast fusion analysis auxotroph. data indicate that C. 10261 is diploid, h0G45 homozygous recessive alleles, are heterozygotes, homozygotes produced crossing-over, association between alleles due linkage.