Genetic and molecular characterization of the genes involved in short-chain fatty acid degradation in Escherichia coli: the ato system

• DOI: 10.1128/jb.169.1.42-52.1987
• Publication Date: 2016-11-02T20:28:24Z
• Authors: L S Jenkins; W D Nunn
• Keywords: Structural gene

The structural organization and regulation of the genes involved in short-chain fatty acid degradation Escherichia coli, referred to as ato system, have been studied by a combination classic genetic recombinant DNA techniques. A plasmid containing 6.2-kilobase region E. coli chromosome was able complement mutations genes, atoA (acetyl-coenzyme [CoA]:acetoacetyl [AA]-CoA transferase) atoB (thiolase II), well regulatory locus, atoC. Complementation studies performed with mutants defective acetyl-CoA:AA-CoA transferase suggest that two loci, atoD atoA, are required for expression functional AA-CoA transferase. gene products were identified vitro transcription translation maxicell analysis proteins 48, 26.5, 26, 42 kilodaltons atoC, atoD, atoB, respectively. In insertional mutagenesis hybrid indicated arranged an operon, order atoD-atoA-atoB. Although transcribed same direction atoDAB atoC appeared use promoter which distinct from used operon. delta expressed only strains gene. exact mechanism control not evident these studies, data product is activator synthesis or activation atoDAB-encoded enzymes.