Isolation of Vibrio harveyi acyl carrier protein and the fabG, acpP, and fabF genes involved in fatty acid biosynthesis

• DOI: 10.1128/jb.178.2.571-573.1996
• Publication Date: 2016-11-10T13:22:21Z
• Authors: Z Shen; D M Byers
• Keywords: Acyl carrier protein; Vibrio harveyi; Fatty acid synthesis

We report the isolation of Vibrio harveyi acyl carrier protein (ACP) and cloning a 3,973-bp region containing fabG (encoding 3-ketoacyl-ACP reductase, 25.5 kDa), acpP ACP, 8.7 fabF synthase II, 43.1 pabC aminodeoxychorismate lyase, 29.9 kDa) genes. Predicted amino acid sequences were, respectively, 78, 86, 76, 35% identical to those corresponding Escherichia coli proteins. Five 11 sequence differences between V. E. ACP were nonconservative concentrated in loop helices I II.