EpsR Modulates Production of Extracellular Polysaccharides in the Bacterial Wilt Pathogen Ralstonia ( Pseudomonas ) solanacearum

• DOI: 10.1128/jb.180.1.27-34.1998
• Publication Date: 2019-12-31T16:22:43Z
• Authors: Matt R. Chapman; C. Cheng Kao
• Keywords: Ralstonia solanacearum; Bacterial wilt; Extracellular polysaccharide; Ralstonia; Pseudomonadales; Opportunistic pathogen

ABSTRACT Ralstonia solanacearum is the causal agent of bacterial wilt many agriculturally important crops. Exopolysaccharide synthesized by products epsI operon major virulence factor for R. . Expression has been demonstrated to be under control several proteins, including two-component regulators. Overexpression EpsR was found previously reduce amount synthesis specifically from promoter. Here we present data that a single chromosomal copy epsR activates promoter, suggesting concentration-dependent effector gene expression. Furthermore, ability modulate expression dependent on phosphorylation state EpsR. Gel mobility shift assays suggest can bind promoter and this binding requires phosphorylated form