Regulation of Acetyl Coenzyme A Synthetase in Escherichia coli

• DOI: 10.1128/jb.182.15.4173-4179.2000
• Publication Date: 2002-07-27T10:00:58Z
• Authors: Suman Kumari; Christine M. Beatty; Douglas F. Browning; Stephen J. W. Busby; Erica J. Simel; Galadriel Hovel-M...; Alan J. Wolfe
• Keywords: Coenzyme A

Cells of Escherichia coli growing on sugars that result in catabolite repression or amino acids feed into glycolysis undergo a metabolic switch associated with the production and utilization acetate. As they divide exponentially, these cells excrete acetate via phosphotransacetylase-acetate kinase pathway. begin transition to stationary phase, instead resorb acetate, activate it acetyl coenzyme A (acetyl-CoA) by means enzyme acetyl-CoA synthetase (Acs) utilize generate energy biosynthetic components tricarboxylic acid cycle glyoxylate shunt, respectively. Here, we present evidence this occurs primarily through induction acs timing magnitude depend, part, direct action carbon regulator cyclic AMP receptor protein (CRP) oxygen FNR. It also depends, probably indirectly, upon shunt repressor IclR, its activator FadR, many enzymes involved metabolism. On basis results, propose induce acs, thus their ability assimilate response rising levels, falling partial pressure, flux acetate-associated pathways.