Towards Single-Copy Gene Expression Systems Making Gene Cloning Physiologically Relevant: Lambda InCh, a Simple Escherichia coli Plasmid-Chromosome Shuttle System

• DOI: 10.1128/jb.182.3.842-847.2000
• Publication Date: 2002-07-27T10:01:09Z
• Authors: Dana Boyd; David S. Weiss; Joseph C. Chen; Jon Beckwith
• Keywords: Lambda phage; Cloning vector; Multiple cloning site; Shuttle vector

ABSTRACT We describe a simple system for reversible, stable integration of plasmid-borne genes into the Escherichia coli chromosome. Most ordinary E. strains and variety pBR322-derived ampicillin-resistant plasmids can be used. A single genetic element, lambda phage, is only specialized vector required. The resultant have copy plasmid fragment inserted stably at attachment site on chromosome, with nearly entire genome deleted.