FosB, a Cysteine-Dependent Fosfomycin Resistance Protein under the Control of ς W , an Extracytoplasmic-Function ς Factor in Bacillus subtilis

• DOI: 10.1128/jb.183.7.2380-2383.2001
• Publication Date: 2002-07-27T10:01:09Z
• Authors: Min Cao; Bryan A. Bernat; Zhepeng Wang; Richard N. Armstrong; John D. Helmann
• Keywords: 0301 basic medicine; Manganese; Transcription, Genetic; Molecular Sequence Data; Drug Resistance, Microbial; Sigma Factor; 03 medical and health sciences; Bacterial Proteins; Fosfomycin; Escherichia coli; Magnesium; Amino Acid Sequence; Cysteine; Bacillus subtilis; Glutathione Transferase

ABSTRACT

We demonstrate that the Bacillus subtilis fosB(yndN) gene encodes a fosfomycin resistance protein. Expression of fosB requires ς W , and both fosB and sigW mutants are fosfomycin sensitive. FosB is a metallothiol transferase related to the FosA class of Mn 2+ -dependent glutathione transferases but with a preference for Mg 2+ and l -cysteine as cofactors.