VmrA, a Member of a Novel Class of Na + -Coupled Multidrug Efflux Pumps from Vibrio parahaemolyticus

• DOI: 10.1128/jb.184.2.572-576.2002
• Publication Date: 2002-07-27T10:03:59Z
• Authors: Jing Chen; Yuji Morita; M. Nazmul Huda; Teruo Kuroda; Tohru Mizushima; Tomofusa Tsuchiya
• Keywords: Efflux; Acriflavine; Ethidium bromide

ABSTRACT Gene vmrA , cloned from Vibrio parahaemolyticus made Escherichia coli resistant to 4prime;,6-diamino-2-phenylindol, tetraphenylphosphonium chloride, acriflavine, and ethidium bromide. VmrA belongs the DinF branch of MATE family efflux transporters. catalyzed acriflavine showed Na + /drug transporter activity because addition -loaded cells caused efflux.