The Protein Encoded at the 3′ End of the Serine Protease Gene of Aeromonas sobria Functions as a Chaperone in the Production of the Protease

• DOI: 10.1128/jb.184.24.7058-7061.2002
• Publication Date: 2002-11-22T18:42:05Z
• Authors: Tomohiko Nomura; Yoshio Fujii; Hiroyasu Yamanaka; Hidetomo Kobayashi; Keinosuke Okamoto
• Keywords: 0303 health sciences; 03 medical and health sciences; Bacterial Proteins; Operon; Serine Endopeptidases; Aeromonas; Molecular Chaperones

ABSTRACT

For the successful production of Aeromonas sobria serine protease (ASP), open reading frame 2 (ORF2) protein, encoded at the 3′ end of the protease operon, is required. In this study, we examined the action of ORF2 protein. The results showed that the protein associated with ASP in the periplasm and helped ASP to form an active structure.