DNA Microarray Analysis of Redox-Responsive Genes in the Genome of the Cyanobacterium Synechocystis sp. Strain PCC 6803

• DOI: 10.1128/jb.185.5.1719-1725.2003
• Publication Date: 2003-02-18T23:46:40Z
• Authors: Yukako Hihara; Kintake Sonoike; Minoru Kanehisa; Masahiko Ikeuchi
• Keywords: Synechocystis; Plastoquinone; DCMU; Strain (injury)

Whole-genome DNA microarrays were used to evaluate the effect of redox state photosynthetic electron transport chain on gene expression in Synechocystis sp. strain PCC 6803. Two specific inhibitors transport, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB), added cultures, changes accumulation transcripts examined. About 140 genes highlighted as reproducibly affected by change chain. It was shown that some stress-responsive but not under control plastoquinone pool