Evidence that the Essential Response Regulator YycF inStreptococcus pneumoniaeModulates Expression of Fatty Acid Biosynthesis Genes and Alters Membrane Composition

• DOI: 10.1128/jb.187.7.2357-2367.2005
• Publication Date: 2005-03-17T21:41:33Z
• Authors: M. Luz Mohedano; Karin Overweg; Alicia de la Fuente; Mark Reuter; Silvia Altabe; Francis Mulholland; Diego de Mendoza; Paloma López; Jerry M. Wells
• Keywords: Regulon; Response regulator; Two-component regulatory system

The YycFG two-component system, originally identified in Bacillus subtilis, is highly conserved among gram-positive bacteria with low G+C contents. In Streptococcus pneumoniae, the YycF response regulator has been reported to be essential for cell growth, but signal which it responds and gene members of regulon remain unclear. order investigate role S. we increased expression yycF by using a maltose-inducible vector analyzed genome-wide effects on transcription protein during course expression. induction histidine kinase yycG transcript levels, suggesting an autoregulation yycFG operon. Evidence from both proteomic microarray transcriptome studies as well analyses membrane fatty acid composition indicated that involved regulation biosynthesis pathways determining chain lengths lipids. agreement recent data pneumococcal cells depleted YycFG, also several other potential are required virulence wall metabolism.