Staphylococcus epidermidis Polysaccharide Intercellular Adhesin Production Significantly Increases during Tricarboxylic Acid Cycle Stress
Aconitate Hydratase
0301 basic medicine
Citric Acid Cycle
Polysaccharides, Bacterial
Gene Expression Regulation, Bacterial
NAD
Adaptation, Physiological
Isocitrate Dehydrogenase
03 medical and health sciences
Glucose
Staphylococcus epidermidis
Biomass
Citrates
Oxidation-Reduction
Acetic Acid
DOI:
10.1128/jb.187.9.2967-2973.2005
Publication Date:
2005-04-18T21:47:59Z
AUTHORS (6)
ABSTRACT
ABSTRACT
Staphylococcal polysaccharide intercellular adhesin (PIA) is important for the development of a mature biofilm. PIA production is increased during growth in a nutrient-replete or iron-limited medium and under conditions of low oxygen availability. Additionally, stress-inducing stimuli such as heat, ethanol, and high concentrations of salt increase the production of PIA. These same environmental conditions are known to repress tricarboxylic acid (TCA) cycle activity, leading us to hypothesize that altering TCA cycle activity would affect PIA production. Culturing
Staphylococcus epidermidis
with a low concentration of the TCA cycle inhibitor fluorocitrate dramatically increased PIA production without impairing glucose catabolism, the growth rate, or the growth yields. These data lead us to speculate that one mechanism by which staphylococci perceive external environmental change is through alterations in TCA cycle activity leading to changes in the intracellular levels of biosynthetic intermediates, ATP, or the redox status of the cell. These changes in the metabolic status of the bacteria result in the attenuation or augmentation of PIA production.
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