Preclinical Diagnosis of Scrapie by Immunohistochemistry of Third Eyelid Lymphoid Tissue

• DOI: 10.1128/jcm.38.9.3254-3259.2000
• Publication Date: 2019-12-31T17:58:41Z
• Authors: K. I. O'Rourke; T. V. Baszler; T. E. Besser; J. M. Miller; R. C. Cutlip; G. A. H. Wells; S. J. Ryder; S. M. Parish; A. N. Hamir; N. E. Cockett; A. Jenny; D. P. Knowles
• Keywords: Sheep; PrPSc Proteins; Lymphoid Tissue; Palatine Tonsil; 610; Antibodies, Monoclonal; 04 agricultural and veterinary sciences; Immunohistochemistry; 630; Clinical Veterinary Microbiology; 3. Good health; 0403 veterinary science; Animals; Nictitating Membrane; Scrapie

Ovine scrapie is a member of the transmissible spongiform encephalopathies (TSEs), heterogeneous family fatal neurologic disorders characterized by deposition an abnormal isoform (prion protein [PrP] PrP-Sc) cellular sialoglycoprotein in neural tissue. PrP-Sc detectable some lymphoid tissues infected sheep months or years before development clinical disease. Detection these basis for live-animal testing. In this study, we characterize performance preclinical diagnostic test ovine based on monoclonal antibody (MAb)-based immunohistochemistry assay nictitating membrane (“third eyelid”)-associated The results third eyelid agreed with status 41 42 suspects confirmed and 174 175 without scrapie. Third sampling 36 clinically normal positive immunostaining brain tissue, including 27 biopsy specimens that progressed to disease 3 20 after biopsy. used MAb F89/160.1.5, which binds residues 142 145 PrP. This can be combination F99/97.6.1, 220 225. One both MAbs cocktail recognize PrP sequences conserved most mammalian species natural TSEs have been reported. Immunohistochemistry routinely formalin-fixed pan-specific practical, readily standardized