Preclinical Diagnosis of African Swine Fever in Contact-Exposed Swine by a Real-Time PCR Assay

• DOI: 10.1128/jcm.43.1.112-119.2005
• Publication Date: 2005-01-05T20:14:18Z
• Authors: L. Zsak; M. V. Borca; G. R. Risatti; A. Zsak; R. A. French; Z. Lu; G. F. Kutish; J. G. Neilan; J. D. Callahan; W. M. Nelson; D. L. Rock
• Keywords: TaqMan; African Swine Fever; Tonsil

ABSTRACT A fluorogenic probe hydrolysis (TaqMan) PCR assay for African swine fever virus (ASFV) was developed and evaluated in experimentally infected swine. This sensitive specific one-step single-tube assay, which can be performed 2 h or less, detected viral DNA tonsil scraping samples to 4 days prior onset of clinical disease. Thus, the would have application preclinical diagnosis surveillance and/or emergency management a disease outbreak.