Identification of Variable-Number Tandem-Repeat Loci in Leptospira interrogans Sensu Stricto

• DOI: 10.1128/jcm.43.2.539-545.2005
• Publication Date: 2005-02-04T21:42:56Z
• Authors: Z. Majed; E. Bellenger; D. Postic; C. Pourcel; G. Baranton; M. Picardeau
• Keywords: Leptospira interrogans

Leptospira interrogans sensu stricto is responsible for the most frequent and severe cases of human leptospirosis. The epidemiology clinical features leptospirosis are usually associated with serovars serogroups Leptospira. Because difficulties serological identification strains, we evaluated a novel PCR-based method typing L. serovars. Based upon genome sequence serovar Lai type strain 5660, 44 loci were analyzed by PCR their variability in size due to presence variable-number tandem repeats (VNTR). Seven VNTR found be powerful markers identification, epidemiology, phylogenetic studies interrogans. This rapid easy should greatly contribute better knowledge