Morphological, Biochemical, and Functional Study of Viral Replication Compartments Isolated from Adenovirus-Infected Cells

• DOI: 10.1128/jvi.00033-16
• Publication Date: 2016-01-14T09:42:57Z
• Authors: Paloma Hidalgo; Lourdes Anzures; Armando Hernández...; Adán Guerrero; Christopher D. Wood; Margarita Valdés; Thomas Dobner; Ramón A. Gonzalez
• Keywords: Nucleoplasm

Adenovirus (Ad) replication compartments (RC) are nuclear microenvironments where the viral genome is replicated and a coordinated program of late gene expression established. These virus-induced sites seem to behave as central hubs for regulation virus-host cell interactions, since proteins that promote efficient well factors participate in antiviral response coopted concentrated there. To gain further insight into activities RC, here we report, first time, morphology, composition, RC isolated from Ad-infected cells. Morphological analyses particles by superresolution microscopy showed they were indistinguishable within infected cells displayed dynamic compartmentalization. Furthermore, RC-containing fractions (RCf) proved be functional, directed de novo synthesis DNA RNA splicing, associated with vivo. A detailed analysis production mRNA RCf at different times postinfection revealed splicing occurs synthesis, posttranscriptional processing, release nucleoplasm individual transcripts spatiotemporally separate events. The results presented demonstrate powerful system study structure, and, result, determination molecular mechanisms induce formation these adenoviruses other nuclear-replicating viruses.RC may represent many aspects interaction controlled. Here, show have morphologies similar those appear compartmentalized, nucleolin DBP display localization periphery sites. direct mRNA, allowing expression. subject temporal patterns regulation, their nucleoplasm. Hence, novel orchestrated take control an cycle.