Characterization of H1N1 Swine Influenza Viruses Circulating in Canadian Pigs in 2009

• DOI: 10.1128/jvi.00801-11
• Publication Date: 2011-06-23T06:43:42Z
• Authors: Charles K. Nfon; Yohannes Berhane; Tamiko Hisanaga; Shunzhen Zhang; Katherine Handel; Helen Kehler; Olivia Labrecque; Nicola S. Lewis; Amy L. Vincent; John Copps; Soren Alexandersen; John Pasick
• Keywords: Reassortment; Pandemic; Oseltamivir; Antigenic shift; H5N1 genetic structure

The 2009 pandemic H1N1 (pH1N1), of apparent swine origin, may have evolved in pigs unnoticed because insufficient surveillance. Consequently, the need for surveillance influenza viruses circulating has received added attention. In this study we characterized isolated from Canadian 2009. Isolates May were comprised hemagglutinin and neuraminidase (NA) genes classical SIV origin combination with North American triple-reassortant internal gene (TRIG) cassette, here termed contemporary (conSIV) H1N1. These conSIV contiguous αH1 cluster, which was distinct pH1N1 isolates that antigenically more related to γH1 cluster. After initial isolation an Alberta pig farm early 2009, found several times pigs. genetically homogeneous. addition, bearing seasonal human H1 N1 together TRIG cassette NA encoding oseltamivir-resistance marker NS one these human-like (shSIV) homologous NS, implicating reassortment between two strains. Antigenic cross-reactivity observed but not shSIV summary, although there cocirculation after no evidence supporting presence prior possibility further reassortants being generated exists should be closely monitored.