Classic Nuclear Localization Signals and a Novel Nuclear Localization Motif Are Required for Nuclear Transport of Porcine Parvovirus Capsid Proteins

• DOI: 10.1128/jvi.01717-14
• Publication Date: 2014-07-31T03:38:54Z
• Authors: Maude Boisvert; Véronique Bouchar...; Sandra Fernandes; Peter Tijssen
• Keywords: NLS; Nuclear lamina; Nuclear pore

ABSTRACT Nuclear targeting of capsid proteins (VPs) is important for genome delivery and precedes assembly in the replication cycle porcine parvovirus (PPV). Clusters basic amino acids, corresponding to potential nuclear localization signals (NLS), were found only unique region VP1 (VP1up, part). Of five identified regions (BR), three VP1up: BR1 was a classic Pat7 NLS, combination BR4 BR5 bipartite NLS. These NLS essential viral replication. VP2, major protein, lacked these contained no with more than two acids proximity. However, clusters folded assembled into trimeric structure. Mutagenesis experiments showed that one involved VP2 transport nucleus. This structural termed motif (NLM), located inside thus can be used trimers nucleus late steps infection but not virions initial steps. The VP1up are N-terminal part externalized from during endosomal transit, exposing them early infection. Globally, determinants PPV different those closely related parvoviruses. IMPORTANCE Most DNA viruses use their cycle. Thus, need targeted this cellular compartment at distinct infection: deliver genomes assemble new viruses. depends on recognition stretch by proteins. study reports identification minor protein (VP1) parvovirus. (VP2) shown depend complex motif. as strategy virus avoid incorrectly selectively import Structural motifs also without acid stretch, including multimeric