Effects of Simultaneous Deletion of pUL11 and Glycoprotein M on Virion Maturation of Herpes Simplex Virus Type 1

• DOI: 10.1128/jvi.01842-08
• Publication Date: 2008-11-13T01:34:56Z
• Authors: Tobias Leege; Walter Fuchs; Harald Granzow; Martina Kopp; Barbara G. Klupp; Thomas C. Mettenl...
• Keywords: Viral tegument; Vero cell; clone (Java method); Virus quantification

ABSTRACT The conserved membrane-associated tegument protein pUL11 and envelope glycoprotein M (gM) are involved in secondary envelopment of herpesvirus nucleocapsids the cytoplasm. Although deletion either gene had only moderate effects on replication related alphaherpesviruses herpes simplex virus type 1 (HSV-1) pseudorabies (PrV) cell culture, simultaneous both genes resulted a severe impairment virion morphogenesis PrV coinciding with formation huge inclusions cytoplasm containing embedded (M. Kopp, H. Granzow, W. Fuchs, B. G. Klupp, T. C. Mettenleiter, J. Virol. 78:3024-3034, 2004). To test whether similar phenotype occurs HSV-1, gM double mutant was generated based newly established bacterial artificial chromosome clone HSV-1 strain KOS. Since gM-negative has not been thoroughly investigated ultrastructurally different phenotypes have ascribed to pUL11-negative single mutants were also constructed analyzed. On monkey kidney (Vero) cells, or ca.-fivefold-reduced titers 40- 50%-reduced plaque diameters compared those wild-type KOS, while rabbit (RK13) cells defects more pronounced, resulting ca.-50-fold titer 70% size reduction for mutant. Electron microscopy revealed that absence inhibited, whereas nuclear stages visibly affected, which is line corresponding mutants. Simultaneous led additive growth and, RK13 large intracytoplasmic capsids material, comparable PrV-ΔUL11/gM-infected cells. HSV-1ΔUL11 HSV-1ΔUL11/gM could be partially corrected trans by PrV. Thus, our data indicate exhibit functions cytoplasmic steps assembly.