Dual Function of the pUL7-pUL51 Tegument Protein Complex in Herpes Simplex Virus 1 Infection

• DOI: 10.1128/jvi.02196-16
• Publication Date: 2016-11-17T04:00:46Z
• Authors: Anna Albecka; Danielle J. Owen; Lyudmila Ivanova; Juliane Brun; Rukayya Liman; Laura Davies; M. Firoz Ahmed; Susanna Colaco; Michael Hollinshead; Stephen C. Graham; Colin M. Crump
• Keywords: Gene Expression Regulation, Viral; UL51; DNA Primase; Herpesvirus 1, Human; Viral Matrix Proteins; Viral Proteins; human herpesviruses; 03 medical and health sciences; Chlorocebus aethiops; Animals; Humans; focal adhesion; Vero Cells; 0303 health sciences; Virus Assembly; DNA Helicases; Herpes Simplex; herpes simplex virus; Virus-Cell Interactions; 3. Good health; Protein Transport; HEK293 Cells; UL7; tegument; Multiprotein Complexes; Protein Binding

ABSTRACT The tegument of herpesviruses is a highly complex structural layer between the nucleocapsid and the envelope of virions. Tegument proteins play both structural and regulatory functions during replication and spread, but the interactions and functions of many of these proteins are poorly understood. Here we focus on two tegument proteins from herpes simplex virus 1 (HSV-1), pUL7 and pUL51, which have homologues in all other herpesviruses. We have now identified that HSV-1 pUL7 and pUL51 form a stable and direct protein-protein interaction, their expression levels rely on the presence of each other, and they function as a complex in infected cells. We demonstrate that expression of the pUL7-pUL51 complex is important for efficient HSV-1 assembly and plaque formation. Furthermore, we also discovered that the pUL7-pUL51 complex localizes to focal adhesions at the plasma membrane in both infected cells and in the absence of other viral proteins. The expression of pUL7-pUL51 is important to stabilize focal adhesions and maintain cell morphology in infected cells and cells infected with viruses lacking pUL7 and/or pUL51 round up more rapidly than cells infected with wild-type HSV-1. Our data suggest that, in addition to the previously reported functions in virus assembly and spread for pUL51, the pUL7-pUL51 complex is important for maintaining the attachment of infected cells to their surroundings through modulating the activity of focal adhesion complexes.

IMPORTANCE Herpesviridae is a large family of highly successful human and animal pathogens. Virions of these viruses are composed of many different proteins, most of which are contained within the tegument, a complex structural layer between the nucleocapsid and the envelope within virus particles. Tegument proteins have important roles in assembling virus particles as well as modifying host cells to promote virus replication and spread. However, little is known about the function of many tegument proteins during virus replication. Our study focuses on two tegument proteins from herpes simplex virus 1 that are conserved in all herpesviruses: pUL7 and pUL51. We demonstrate that these proteins directly interact and form a functional complex that is important for both virus assembly and modulation of host cell morphology. Further, we identify for the first time that these conserved herpesvirus tegument proteins localize to focal adhesions in addition to cytoplasmic juxtanuclear membranes within infected cells.