Full Genome-Based Classification of Rotaviruses Reveals a Common Origin between Human Wa-Like and Porcine Rotavirus Strains and Human DS-1-Like and Bovine Rotavirus Strains

• DOI: 10.1128/jvi.02257-07
• Publication Date: 2008-01-24T01:34:06Z
• Authors: Jelle Matthijnssens; Max Ciarlet; Erica Heiman; Ingrid Arijs; Thomas Delbeke; Sarah M. McDonald; Enzo A. Palombo; Miren Iturriza-Go...; Piet Maes; John T. Patton; Mustafizur Rahman; Marc Van Ranst
• Keywords: Reassortment; Molecular Epidemiology

ABSTRACT Group A rotavirus classification is currently based on the molecular properties of two outer layer proteins, VP7 and VP4, middle protein, VP6. As reassortment all 11 gene segments plays a key role in generating diversity nature, system that desirable for determining which genes influence host range restriction, replication, virulence, as well studying epidemiology evolution. Toward establishing such system, sequences encoding VP1 to VP3, VP6, NSP1 NSP5 were determined human animal strains belonging different G P genotypes addition those available databases, they used define phylogenetic relationships among genes. Based these analyses, appropriate identity cutoff values each gene. For VP4 gene, nucleotide value 80% completely correlated with 27 established genotypes. largely coincided but identified four additional distinct comprised murine or avian strains. Phylogenetic analyses showed existence 4, 5, 6, 11, 14, 7, 6 genotypes, respectively, 83%, 84%, 81%, 85%, 79%, 91%, respectively. In accordance data, revised nomenclature proposed. The novel allows identification (i) probably followed separate evolutionary paths; (ii) interspecies transmissions plethora events; (iii) certain constellations revealed (a) common origin between Wa-like porcine (b) DS-1-like bovine rotaviruses. These close links rotaviruses emphasize need simultaneous monitoring animals humans.