Human Annexin A6 Interacts with Influenza A Virus Protein M2 and Negatively Modulates Infection

• DOI: 10.1128/jvi.06003-11
• Publication Date: 2011-11-24T05:47:49Z
• Authors: Huailiang Ma; François Kien; Maxime Manière; Yang Zhang; Nadège Lagarde; Kong San Tse; Leo Lit Man Poon; Béatrice Nal
• Keywords: Viral life cycle; RNA virus

ABSTRACT The influenza A virus M2 ion channel protein has the longest cytoplasmic tail (CT) among three viral envelope proteins and is well conserved between different strains. It accessible to host cellular machinery after fusion with endosomal membrane during trafficking, assembly, budding processes. We hypothesized that identification of interactants CT could help us better understand molecular mechanisms regulating M2-dependent stages life cycle. Using yeast two-hybrid screening as bait, a novel interaction human annexin A6 (AnxA6) was identified, their physical confirmed by coimmunoprecipitation assay colocalization study virus-infected cells. found small interfering RNA (siRNA)-mediated knockdown AnxA6 expression significantly increased production, while its overexpression reduce titer progeny, suggesting negative regulatory role for infection. Further characterization revealed depletion or had no effect on early cycle replication but impaired release progeny virus, suggested delayed defective events observed at plasma cells transmission electron microscopy. Collectively, this work identifies regulator targets impairs