Hsp70 Protein Positively Regulates Rabies Virus Infection

• DOI: 10.1128/jvi.06501-11
• Publication Date: 2012-02-16T06:57:25Z
• Authors: Xavier Lahaye; Aurore Vidy; Baptiste Fouquet; Danielle Blondel
• Keywords: Viral structural protein; Nucleoprotein; Transcription; Viral protein; Viral life cycle; Chaperone (clinical); Mononegavirales

The Hsp70 chaperone plays a central role in multiple processes within cells, including protein translation, folding, intracellular trafficking, and degradation. This is implicated the replication of numerous viruses. We have shown that rabies virus infection induced cellular expression Hsp70, which accumulated Negri body-like structures, where viral transcription take place. In addition, present both nucleocapsids purified from infected cells virions. has been to interact with nucleoprotein N. downregulation using specific inhibitors, such as quercetin or RNA interference, resulted significant decrease amount mRNAs, proteins, particles. These results indicate proviral function during suggest involved at least one stage(s) life cycle, transcription, and/or production. mechanism by controls will be discussed.