Temporal patterns of human immunodeficiency virus type 1 transcripts in human fetal astrocytes

• DOI: 10.1128/jvi.68.1.93-102.1994
• Publication Date: 2020-01-06T16:41:24Z
• Authors: C Tornatore; K Meyers; W Atwood; K Conant; E Major
• Keywords: Subgenomic mRNA; Virus latency

Human immunodeficiency virus type 1 (HIV-1) infection of the developing central nervous system results in a dementing process children, termed HIV-1-associated encephalopathy. Infection astroglial elements pediatric has been demonstrated and suggests that direct some astrocytes may contribute to neurologic deficit. In this model, HIV-1 establishes persistent state astrocytes, which can be reactivated by cytokines tumor necrosis factor alpha (TNF-alpha) interleukin beta (IL-1 beta). To better understand natural history viral persistence cells, we characterized at transcriptional level. The most abundant transcript during establishment was subgenomic multiply spliced 2-kb message, similar mononuclear cell models latency. Following reactivation with TNF-alpha or IL-1 message remained transcript, contrast infected cells leads reemergence 9- 4-kb transcripts. Further characterization PCR amplification vitro-synthesized cDNA showed that, absence cytokine stimulation, transcripts were Nef- Rev-specific messages. However, following double- triple-spliced Tat-, Rev-, Nef-specific messages could identified. Immunohistochemical staining persistence, expressed Nef protein but few no structural proteins. These demonstrate level is fundamentally different from seen account for virtual expression antigens vivo.