The minimal conserved transcription stop-start signal promotes stable expression of a foreign gene in vesicular stomatitis virus

• DOI: 10.1128/jvi.70.4.2318-2323.1996
• Publication Date: 2020-01-06T21:46:02Z
• Authors: M J Schnell; L Buonocore; M A Whitt; J K Rose
• Keywords: Transcription; Chloramphenicol acetyltransferase; Terminator (solar)

A new transcription unit was generated in the 3' noncoding region of vesicular stomatitis virus (VSV) glycoprotein gene by introducing smallest conserved sequence found at each VSV junction. This introduced into a DNA copy genome from which infectious can be derived. It contained an 11-nucleotide putative stop/polyadenylation signal for mRNA, intergenic dinucleotide, and 10-nucleotide start preceding downstream foreign encoding bacterial enzyme chloramphenicol acetyltransferase. Infectious recombinant recovered this construct to express high levels functional acetyltransferase mRNA protein. The grew wild-type titers 5 x 10(9)/ml, expression completely stable least 15 passages involving 10(6)-fold expansion passage. These results define functionally sequences also illustrate utility as vector that should have wide application cell biology vaccine development.