Rapid Selection in Modified BHK-21 Cells of a Foot-and-Mouth Disease Virus Variant Showing Alterations in Cell Tropism

• DOI: 10.1128/jvi.72.12.10171-10179.1998
• Publication Date: 2019-12-31T18:06:25Z
• Authors: Cristina Escarmís; Elisa C. Carrillo; Marcela Ferrer; Juan F. García A...; Nora Lopez; Cecilia Tami; Nuria Verdaguer; Esteban Domingo; Maria T. Franze-F...
• Keywords: Tissue tropism

With persistent foot-and-mouth disease virus (FMDV) in BHK-21 cells, there is coevolution of the cells and resident virus; virulence for parental gradually increased, become partially resistant to FMDV. Here we report that variants FMDV C3Arg/85 were selected a single infection (termed BHK-Rb cells). Indirect immunofluorescence showed cell population was heterogeneous with regard susceptibility infection. Infection resulted an early phase partial cytopathology which followed at 6 10 days postinfection by shedding mutant FMDVs, termed C3-Rb. The C3-Rb increased able overcome resistance modified infection, had acquired ability bind heparin infect wild-type Chinese hamster ovary (CHO) cells. A comparison genomic sequences viruses revealed only two amino acid differences, located surface particle, fivefold axis viral capsid (Asp-9-->Ala VP3 either Gly-110-->Arg or His-108-->Arg VP1). same phenotypic genotypic modifications occurred highly reproducible manner; they seen number independent infections preparation clones derived from it. Neither substitutions other structural nonstructural proteins nor nucleotide regulatory regions found. These results prove permissive can promote rapid selection show alterations tropism are virulent