Identification of a Full-Length cDNA for an Endogenous Retrovirus of Miniature Swine

• DOI: 10.1128/jvi.72.5.4503-4507.1998
• Publication Date: 2019-12-31T18:13:15Z
• Authors: Donna E. Akiyoshi; Maria Denaro; Haihong Zhu; Julia L. Greenstein; Papia Banerjee; Jay A. Fishman
• Keywords: Endogenous retrovirus; Xenotransplantation; Miniature swine; Gammaretrovirus; Feline leukemia virus; Bovine leukemia virus

ABSTRACT Endogenous retroviruses of swine are a concern in the use pig-derived tissues for xenotransplantation into humans. The nucleotide sequence porcine endogenous retrovirus taken from lymphocytes miniature (PERV-MSL) has been characterized. PERV-MSL is type C 8,132 bp with greatest nucleic acid identity to gibbon ape leukemia virus and murine virus. Constitutive production RNA detected normal leukocytes multiple organs swine. copy numbers full-length PERV sequences per genome (approximately 8 15) vary among strains. open reading frames gag , pol env have over 99% amino those Tsukuba-1 highly homologous cell line PK15 (PK15-ERV). Most differences predicted PK15-ERV SU (cell attach- ment) region . existence these clones will enable studies infection by xenotransplantation.