vig-1, a New Fish Gene Induced by the Rhabdovirus Glycoprotein, Has a Virus-Induced Homologue in Humans and Shares Conserved Motifs with the MoaA Family

• DOI: 10.1128/jvi.73.3.1846-1852.1999
• Publication Date: 2019-12-31T18:24:04Z
• Authors: Pierre Boudinot; Pascale Massin; Mar Blanco; Sabine Riffault; Abdenour Benmansour
• Keywords: Mononegavirales

ABSTRACT We used mRNA differential display methodology to analyze the shift of transcription profile induced by fish rhabdovirus, viral hemorrhagic septicemia virus (VHSV), in rainbow trout leukocytes. identified and characterized a new gene which is directly VHSV. This VHSV-induced ( vig-1 ) encodes 348-amino-acid protein. highly expressed during experimental disease lymphoid organs infected fish. Intramuscular injection plasmid vector expressing glycoprotein results expression, showing that external protein sufficient for induction. expression also obtained interferon-like factor, indicating can be through different pathways. Moreover, homologous recently described human cytomegalovirus-induced gene. Accordingly, activation may represent virus-induced pathway conserved vertebrates. The deduced amino acid sequence significantly related sequences required biosynthesis metal cofactors. suggests function involved nonspecific synthesis enzymatic cofactors nitric oxide pathway.