Sendai Virus and Simian Virus 5 Block Activation of Interferon-Responsive Genes: Importance for Virus Pathogenesis

• DOI: 10.1128/jvi.73.4.3125-3133.1999
• Publication Date: 2019-12-31T18:25:46Z
• Authors: L. Didcock; D. F. Young; S. Goodbourn; R. E. Randall
• Keywords: Sendai virus; Pathogenesis; Mononegavirales

ABSTRACT Sendai virus (SeV) is highly pathogenic for mice. In contrast, mice (including SCID mice) infected with simian 5 (SV5) showed no overt signs of disease. Evidence presented that a major factor which prevented SV5 from productively infecting was its inability to circumvent the interferon (IFN) response in Thus, murine cells produce and respond IFN, protein synthesis rapidly switched off. marked once SeV began, it continued, even if culture medium supplemented alpha/beta IFN (IFN-α/β). However, human cells, IFN-α/β did not inhibit replication either or established. To begin address molecular basis these observations, effects infections on activation an IFN-α/β-responsive promoter IFN-β were examined transient transfection experiments. The results demonstrated (i) SeV, but SV5, inhibited cells; (ii) both (iii) strong inducer promoter, whereas poor inducer. ability IFN-responsive genes confirmed by RNase protection importance terms paramyxovirus pathogenesis discussed.