Centrosome Abnormalities and Genomic Instability by Episomal Expression of Human Papillomavirus Type 16 in Raft Cultures of Human Keratinocytes

• DOI: 10.1128/jvi.75.16.7712-7716.2001
• Publication Date: 2002-07-27T09:58:35Z
• Authors: Stefan Duensing; Anette Duensing; Elsa R. Flores; Anh Do; Paul F. Lambert; Karl Münger
• Keywords: Ectopic expression

ABSTRACT Primary human keratinocytes with ectopic expression of high-risk papillomavirus (HPV) E6 and E7 oncoproteins display abnormal centrosome numbers, multipolar mitoses, aneusomy. However, it has not been explored whether these abnormalities can occur in cells containing HPV episomes where is under viral transcriptional control. Here, we demonstrate that genomic instability organotypic raft cultures episomal HPV-16 even at low copy numbers. We conclude DNA, when maintained as an episome, disturb homeostasis subvert integrity the host cell during early stages infection.