Identification of BV/ODV-C42, an Autographa californica Nucleopolyhedrovirus orf101 -Encoded Structural Protein Detected in Infected-Cell Complexes with ODV-EC27 and p78/83

• DOI: 10.1128/jvi.75.24.12331-12338.2001
• Publication Date: 2002-07-27T10:06:37Z
• Authors: Sharon C. Braunagel; Paula A. Guidry; German Rosas-Acosta; Luke Engelking; Max D. Summers
• Keywords: Autographa californica

orf101 is a late gene of Autographa californica nucleopolyhedrovirus (AcMNPV). It encodes protein 42 kDa which component the nucleocapsid budded virus (BV) and occlusion-derived (ODV). To reflect this viral localization, product was named BV/ODV-C42 (C42). C42 predominantly detected within infected-cell nucleus: at 24 h postinfection (p.i.), it coincident with virogenic stroma, but by 72 p.i., stroma minimally labeled while more uniformly located throughout nucleus. Yeast two-hybrid screens indicate that capable directly interacting proteins p78/83 (1629K) ODV-EC27 (orf144). These interactions were confirmed using blue native gels Western blot analyses. At 28 are in two complexes: one approximately 180 high-molecular-mass complex (500 to 600 kDa) also contains EC27.