Susceptibility of Immature and Mature Langerhans Cell-Type Dendritic Cells to Infection and Immunomodulation by Human Cytomegalovirus

• DOI: 10.1128/jvi.77.13.7563-7574.2003
• Publication Date: 2003-06-13T00:18:39Z
• Authors: Laura Hertel; Vashti G. Lacaille; Herbert Strobl; Elizabeth D. Mellins; Edward S. Mocarski
• Keywords: Cytomegalovirus; Cell type

ABSTRACT Human cytomegalovirus (CMV) infection initiates in mucosal epithelia and disseminates via leukocytes throughout the body. Langerhans cells (LCs), immature dendritic (DCs) that reside epithelial tissues, are among first to encounter virus may play important roles immune response, as well pathogenesis hosts for viral replication vehicles dissemination. Here, we demonstrate CD34 + progenitor cell-derived LC-type DCs exhibit a differentiation state-dependent susceptibility CMV infection. In contrast small percentage (3 4%) of LCs supported infection, high (48 74%) mature, LC-derived were susceptible with endotheliotropic strains (TB40/E or VHL/E) CMV. These much less AD169 var ATCC, Towne RIT 3 , Toledo. When exposed strains, gene expression (IE1/IE2 other products) proceeded efficiently mature (mDCs). Productive was associated downmodulation cell surface CD83, CD1a, CD80, CD86, ICAM-1, major histocompatibility complex (MHC) class I, MHC II on these cells. addition, T-cell proliferative response allogeneic mDCs attenuated when CMV-infected cultures used stimulators. This investigation revealed characteristics interaction between LC lineage DCs, suggesting immunity through their increased virus-mediated escape.