Cellular Proteins from Human Monocytes Bind to Dengue 4 Virus Minus-Strand 3′ Untranslated Region RNA

• DOI: 10.1128/jvi.77.5.3067-3076.2003
• Publication Date: 2003-02-12T20:46:14Z
• Authors: Rosa Martha E. Yo...; Fernando Medina; Jorge Reyes-del V...; Rosa M. del Angel
• Keywords: Immunoprecipitation

The synthesis of plus and minus RNA strands several viruses requires as a first step the interaction some viral regulatory sequences with cellular proteins. dengue 4 virus genome, single-stranded, positive-polarity RNA, is flanked by two untranslated regions (UTR) located in 5' 3' ends. 3'UTR minus-strand [3'UTR (-)] has been thought to function promoter for plus-strand RNA. To study initial between this proteins, mobility shift assays were performed, four ribonucleoprotein complexes (I through IV) formed when uninfected infected U937 cells (human monocyte cell line) interacted (-) virus. Cross-linking RNAs containing complete (nucleotides [nt] 101 1) or partial sequence from nt 45 44 1 resulted specific binding Supermobility immunoprecipitation demonstrated that La protein forms part these complexes. determine region UTR protein, deletion mutants generated. mutant (del-96), 96 101, was unable interact suggesting portion (-). Complex I, which main complex had fastest electrophoretic migration, contained proteins such calreticulin disulfide isomerase, constitute important components endoplasmic reticulum.