Cloning and Characterization of a Bovine Adeno-Associated Virus

• DOI: 10.1128/jvi.78.12.6509-6516.2004
• Publication Date: 2004-05-26T17:12:54Z
• Authors: Michael Schmidt; Hisako Katano; Ioannis Bossis; John A. Chiorini
• Keywords: Transduction (biophysics); Adeno-associated virus; Homology

ABSTRACT To better understand the relationship between primate adeno-associated viruses (AAVs) and those of other mammals, we have cloned sequenced genome an AAV found as a contaminant in two isolates bovine adenovirus that was reported to be serologically distinct from AAVs. The (BAAV) has 4,693 bp, its organization is similar isolates. left-hand open reading frame (ORF) both inverted terminal repeats (ITRs) highest homology with rep ORF ITRs serotype 5 (AAV-5) (89 96%, respectively). However, right-hand only 55% identical AAV-5 capsid ORF; it had AAV-4 (76%). By comparing BAAV cap sequence model capsid, mapped regions VP1 are divergent AAV-4. These located on outside partially exposed loops. not neutralized by antisera raised against recombinant AAV-2, AAV-4, or AAV-5, demonstrated unique cell tropism profile four human cancer lines, suggesting might transduction activity A murine salivary gland gene transfer used evaluate vivo performance BAAV. Recombinant BAAV-mediated 11 times more efficient than AAV-2. Overall, these data suggest vectors based could useful for applications.