Role of JC Virus Agnoprotein in DNA Repair

• DOI: 10.1128/jvi.78.16.8593-8600.2004
• Publication Date: 2004-07-27T18:42:49Z
• Authors: Armine Darbinyan; Khwaja M. Siddiqui; Dorota Slonina; Nune Darbinian; Shohreh Amini; Martyn K. White; Kamel Khalili
• Keywords: Ku70

ABSTRACT The late region of human neurotropic JC virus encodes a small 71-amino-acid agnoprotein that is also found in the polyomaviruses simian 40 and BK virus. Several functions have been identified, including roles regulating viral transcription virion maturation. Earlier studies showed expressed alone induced p21/WAF-1 expression caused cells to accumulate G 2 /M stage cell cycle. Here we report sensitized cytotoxic effects DNA-damaging agent cisplatin. Agnoprotein reduced viability cisplatin-treated increased chromosome fragmentation micronucleus formation. Whereas control accumulated S phase, expressing did not, instead becoming aneuploid. correlated with impaired double-strand-break repair activity cellular extracts Ku70 Ku80 DNA proteins. After expression, much protein was located perinuclear space, where found. Results from binding an interaction which mediated by N terminus. ability inhibit double-strand break when it added We conclude inhibits after damage interferes damage-induced cycle regulation. Since subunit DNA-dependent kinase responsible both for signaling arrest, modulation and/or may represent important event polyomavirus life transformation.