Nitric Oxide as an Endogenous Mutagen for Sendai Virus without Antiviral Activity

• DOI: 10.1128/jvi.78.16.8709-8719.2004
• Publication Date: 2004-07-27T18:42:49Z
• Authors: Jun Yoshitake; Takaaki Akaike; Teruo Akuta; Fumio Tamura; Tsutomu Ogura; Hiroyasu Esumi; Hiroshi Maeda
• Keywords: Sendai virus

Nitric oxide (NO) may affect the genomes of various pathogens, and this mutagenesis is particular interest for viral pathogenesis evolution. Here, we investigated effect NO on replication mutation. Exogenous or endogenous had no apparent antiviral influenza A virus Sendai virus. The mutagenic potential was analyzed with fused to a green fluorescent protein (GFP) gene (GFP-SeV). GFP-SeV cultured in SW480 cells transfected vector expressing inducible synthase (iNOS). mutation frequency examined by measuring loss GFP fluorescence plaques. iNOS-SW480 much higher than that parent reduced level treatment an (NOS) inhibitor. Immunocytochemistry showed generation more 8-nitroguanosine without iNOS transfection. Authentic added exogenously GFP-SeV-infected CV-1 increased frequency. Profiles mutations induced appeared resemble those occurring mouse lungs vivo. base substitution characteristic both mutants (those vivo) C-to-U transition. NO-dependent oxidative stress also evident. Together, results indicate unambiguously has RNA viruses such as affecting replication, possibly via formation cellular stress.