Cyclin-Dependent Kinase Activity Is Required at Early Times for Accurate Processing and Accumulation of the Human Cytomegalovirus UL122-123 and UL37 Immediate-Early Transcripts and at Later Times for Virus Production

• DOI: 10.1128/jvi.78.20.11219-11232.2004
• Publication Date: 2004-09-27T17:28:05Z
• Authors: Veronica Sanchez; Anita K. McElroy; Judy Yen; Sama Tamrakar; Charles L. Clark; Rachel A. Schwartz; Deborah H. Spector
• Keywords: Cytomegalovirus

Human cytomegalovirus (HCMV) infection leads to dysregulation of multiple cell cycle-regulatory proteins. In this study, we examined the effects inhibition cyclin-dependent kinase (cdk) activity on viral replication. With drug Roscovitine, a specific inhibitor kinases 1, 2, 5, 7, and 9, have shown that during first 6 h infection, kinase-dependent events occurred included regulated processing accumulation immediate-early (IE) UL122-123 transcripts UL36-37 transcripts. Altered led loss IE1-72 an increase in IE2-86. The ratio spliced unspliced UL37 also changed. These did not require de novo protein synthesis or degradation proteins by proteasome. Addition Roscovitine at beginning was associated with expression selected early gene products, DNA replication, late expression. When added after IE were no longer observed replication proceeded through phase, but titers reduced. reduction titer even when 48 postinfection, indicating is required both times. Flavopiridol, another kinases, had similar results underscore importance accurate RNA reiterate significant role factors HCMV infection.