Small Molecules Blocking the Entry of Severe Acute Respiratory Syndrome Coronavirus into Host Cells

• DOI: 10.1128/jvi.78.20.11334-11339.2004
• Publication Date: 2004-09-27T17:28:05Z
• Authors: Ling Yi; Zhengquan Li; Kehu Yuan; Xiuxia Qu; Jian Chen; Guangwen Wang; Hong Zhang; Hongpeng Luo; Lili Zhu; Pengfei Jiang; Lirong Chen; Yan Shen; Min Luo; Guoying Zuo; Jianhe Hu; Deliang Duan; Yuchun Nie; Xuanling Shi; Wei Wang; Yang Han; Taisheng Li; Yuqing Liu; Mingxiao Ding; Hongkui Deng; Xiaojie Xu
• Keywords: Coronavirus; Entry inhibitor; Coronaviridae

Severe acute respiratory syndrome coronavirus (SARS-CoV) is the pathogen of SARS, which caused a global panic in 2003. We describe here screening Chinese herbal medicine-based, novel small molecules that bind avidly with surface spike protein SARS-CoV and thus can interfere entry virus to its host cells. achieved this by using two-step method consisting frontal affinity chromatography-mass spectrometry coupled viral infection assay based on human immunodeficiency (HIV)-luc/SARS pseudotyped virus. Two molecules, tetra-O-galloyl-beta-D-glucose (TGG) luteolin, were identified, whose anti-SARS-CoV activities confirmed wild-type system. TGG exhibits prominent activity 50% effective concentration 4.5 microM selective index 240.0. The described yielded several be used for developing new classes drugs potentially useful high-throughput inhibiting HIV, hepatitis C virus, other insidious viruses into their