Lentiviral Vectors Interfering with Virus-Induced CD4 Down-Modulation Potently Block Human Immunodeficiency Virus Type 1 Replication in Primary Lymphocytes

• DOI: 10.1128/jvi.78.23.13072-13081.2004
• Publication Date: 2004-11-12T20:29:30Z
• Authors: Hang M. Pham; Enrique R. Argañ...; Bettina Groschel; Didier Trono; Juan Lama
• Keywords: Infectivity

ABSTRACT CD4 down-modulation is essential for the production of human immunodeficiency virus (HIV) infectious particles. Disease progression correlates with enhanced viral induced down-modulation, and a subset long-term nonprogressors carry viruses defective in this function. Despite multiple pieces evidence highlighting importance function pathogenesis vivo, to date, HIV-induced has not been used as target intervention. We describe here HIV-based vectors that deliver truncated molecules resistant by products Nef Vpu. Infection cells previously transduced these proceeded normally, particles were released normal amounts. However, infectivity virions was reduced 1,000-fold. Lentiviral expressing efficient at blocking HIV-1 replication several cell lines CD4-positive primary lymphocytes. The findings presented provide proof-of-principle approaches targeting virus-induced may constitute basis novel anti-HIV therapies.