CD46 Is a Cellular Receptor for Bovine Viral Diarrhea Virus

• DOI: 10.1128/jvi.78.4.1792-1799.2004
• Publication Date: 2004-01-27T19:58:01Z
• Authors: Karin Maurer; Thomas Krey; Volker Moennig; Heinz-Jürgen Thiel; Till Rümenapf
• Keywords: DNA, Complementary; Diarrhea Viruses, Bovine Viral; Membrane Glycoproteins; Swine; Molecular Sequence Data; Antibodies, Monoclonal; Sequence Analysis, DNA; 3. Good health; Cell Line; Membrane Cofactor Protein; Mice; Antigens, CD; Chlorocebus aethiops; Animals; Humans; Receptors, Virus; Cattle; Amino Acid Sequence; Vero Cells; HeLa Cells

ABSTRACT Various monoclonal antibodies (MAbs) that recognize cell surface proteins on bovine cells were previously shown to efficiently block infection with viral diarrhea virus (BVDV) (C. Schelp, I. Greiser-Wilke, G. Wolf, M. Beer, V. Moennig, and B. Liess, Arch. Virol. 140: 1997-2009, 1995). With one of these MAbs, a 50- 58-kDa protein was purified from calf thymus by immunoaffinity chromatography. Microchemical analysis two internal peptides revealed significant sequence homology porcine human CD46. The cDNA CD46 (CD46 bov ) cloned further characterized. Heterologously expressed detected the MAb used for purification. A putative function as BVDV receptor studied respect binding susceptibility nonpermissive cells. While expression correlated well [ 3 H]uridine-labeled BVDV, not observed. However, resulted in increase BVDV. These results provide strong evidence serves cellular