Adenovirus Virus-Associated RNA Is Processed to Functional Interfering RNAs Involved in Virus Production

• DOI: 10.1128/jvi.80.3.1376-1384.2006
• Publication Date: 2006-01-13T13:18:00Z
• Authors: Oscar Aparicio; Nerea Razquin; Mikel Zaratiegui; Iñigo Narvaiza; Puri Fortes
• Keywords: Argonaute; Trans-acting siRNA; RNA Silencing; Small nucleolar RNA; RNA-induced silencing complex; DNA-directed RNA interference

ABSTRACT Posttranscriptional gene silencing allows sequence-specific control of expression. Specificity is guaranteed by small antisense RNAs such as microRNAs (miRNAs) or interfering (siRNAs). Functional miRNAs derive from longer double-stranded RNA (dsRNA) molecules that are cleaved to pre-miRNAs in the nucleus and transported exportin 5 (Exp 5) cytoplasm. Adenovirus-infected cells express virus-associated (VA) RNAs, which dsRNA similar structure pre-miRNAs. VA also Exp cytoplasm, where they accumulate. Here we show derived (svaRNAs), miRNAs, can be found adenovirus-infected cells. processing svaRNAs requires neither viral replication nor protein expression, evidenced fact svaRNA accumulation detected transfected with sequences. efficiently bound Argonaute 2, endonuclease RNA-induced complex, behave functional siRNAs, inhibit expression reporter genes complementary Blocking svaRNA-mediated inhibition affects efficient adenovirus production, indicating required for virus viability. Thus, could represent a novel mechanism used adenoviruses cellular