A Novel Alternative Splicing Isoform of Human T-Cell Leukemia Virus Type 1 bZIP Factor (HBZ-SI) Targets Distinct Subnuclear Localization

• DOI: 10.1128/jvi.80.5.2495-2505.2006
• Publication Date: 2006-02-11T01:27:20Z
• Authors: Ken Murata; Toshihisa Hayashi...; Kazuyuki Sugahara; Akiko Uemura; Taku Yamaguchi; Hitomi Harasawa; Hiroo Hasegawa; Kazuto Tsuruda; Toshiro Okazaki; Takehiko Koji; Takayuki Miyanishi; Yasuaki Yamada; Shimeru Kamihira
• Keywords: Cell Nucleus; Human T-lymphotropic virus 1; 0303 health sciences; Base Sequence; Reverse Transcriptase Polymerase Chain Reaction; Blotting, Western; Green Fluorescent Proteins; Molecular Sequence Data; Retroviridae Proteins; Biological Transport; Artificial Gene Fusion; 3. Good health; Alternative Splicing; 03 medical and health sciences; Basic-Leucine Zipper Transcription Factors; Microscopy, Fluorescence; Cell Line, Tumor; Humans; Protein Isoforms; RNA, Viral; Amino Acid Sequence; RNA, Messenger; RNA Processing, Post-Transcriptional

Adult T-cell leukemia (ATL) is associated with prior infection human virus type 1 (HTLV-1); however, the mechanism by which HTLV-1 causes adult has not been fully elucidated. Recently, a functional basic leucine zipper (bZIP) protein coded in minus strand of genome (HBZ) was identified. We report here novel isoform bZIP factor (HBZ), HBZ-SI, identified means reverse transcription-PCR (RT-PCR) conjunction 5' and 3' rapid amplification cDNA ends (RACE). HBZ-SI 206-amino-acid-long generated alternative splicing between part HBZ gene exon located long terminal repeat genome. Consequently, these isoforms share >95% amino acid sequence identity, differ only at their N termini, indicating that also protein. Duplex RT-PCR real-time quantitative analyses showed mRNAs were expressed equivalent levels all ATL cell samples examined. Nonetheless, we found Western blotting preferentially some lines A key finding obtained from subcellular localization isoforms. Despite high similarity, each targeted to distinguishable subnuclear structures. These data show presence cells, addition, shed new light on possibility may play unique role distinct regions nucleus.