Botulinum neurotoxin X lacks potency in mice and in human neurons

• DOI: 10.1128/mbio.03106-23
• Publication Date: 2024-02-13T05:00:19Z
• Authors: Brieana M. Gregg; Takuhiro Matsumura; Travis G. Wentz; William H. Tepp; Marite Bradshaw; Pål Stenmark; Eric A. Johnson; Yukako Fujinaga; Sabine Pellett
• Keywords: 0301 basic medicine; 03 medical and health sciences; Clostridium botulinum; botulinum neurotoxin; BoNT; BoNT/X; Microbiology; QR1-502; Research Article

ABSTRACT

Botulinum neurotoxins (BoNTs) are a class of toxins produced by Clostridium botulinum ( C. botulinum ) and other species of Clostridia . BoNT/X is a putative novel botulinum neurotoxin identified through genome sequencing and capable of SNARE cleavage, but its neurotoxic potential in humans and vertebrates remained unclear. The C. botulinum strain producing BoNT/X, Strain 111, encodes both a plasmid-borne bont/b2 as well as the chromosomal putative bont/x . This study utilized C. botulinum Strain 111 from Japan as well as recombinantly produced full-length BoNT/X to more fully analyze this putative pathogenic toxin. We confirmed production of full-length, catalytically active native BoNT/X by C. botulinum Strain 111, produced as a disulfide-bonded dichain polypeptide similar to other BoNTs. Both the purified native and the recombinant BoNT/X had high enzymatic activity in vitro but displayed very low potency in human-induced pluripotent stem cell-derived neuronal cells and in mice. Intraperitoneal injection of up to 50 µg of native BoNT/X in mice did not result in botulism; however, mild local paralysis was observed after injection of 2 μg into the gastrocnemius muscle. We further demonstrate that the lack of toxicity by BoNT/X is due to inefficient neuronal cell association and entry, which can be rescued by replacing the receptor binding domain of BoNT/X with that of BoNT/A. These data demonstrate that BoNT/X is not a potent vertebrate neurotoxin like the classical seven serotypes of BoNTs.

IMPORTANCE The family of botulinum neurotoxins comprises the most potent toxins known to humankind. New members of this family of protein toxins as well as more distantly related homologs are being identified. The discovery of BoNT/X via bioinformatic screen in 2017 as a putative new BoNT serotype raised concern about its potential as a pathogenic agent with no available countermeasures. This study for the first time assessed both recombinantly produced and native purified BoNT/X for its vertebrate neurotoxicity.