Point mutations upstream of the yeast ADH2 poly(A) site significantly reduce the efficiency of 3'-end formation.

• DOI: 10.1128/mcb.11.4.2004
• Publication Date: 2015-10-06T00:24:59Z
• Authors: L E Hyman; S H Seiler; J Whoriskey; C L Moore
• Keywords: Coding region

The sequences directing formation of mRNA 3' ends in Saccharomyces cerevisiae are not well defined. This is contrast to the situation higher eukaryotes which sequence AAUAAA known be crucial proper 3'-end formation. hexanucleotide found upstream poly(A) site some but all yeast genes. One these gene coding for alcohol dehydrogenase, ADH2. Deletion or a double point mutation has only small effect on efficiency reaction, and mammalian system, it most likely operating as major processing signal cell. However, we isolated mutations reveal that region located approximately 80 nucleotides plays critical role either transcription termination, polyadenylation, both. These represent first yeasts significantly reduce