Nucleosome loss activates CUP1 and HIS3 promoters to fully induced levels in the yeast Saccharomyces cerevisiae.

• DOI: 10.1128/mcb.12.4.1621
• Publication Date: 2015-10-06T00:29:18Z
• Authors: L K Durrin; R K Mann; M Grunstein
• Keywords: Transcription

We have previously shown that nucleosome loss, obtained by repressing histone H4 mRNA synthesis, activates otherwise inactive PHO5, GAL1, and CYC1 gene promoters (fused to the bacterial beta-galactosidase [lacZ] reporter gene) moderate levels of activity (approximately 2 15% fully induced levels). now report loss expression two additional are normally independent mechanisms: CUP1 (induced heavy-metal toxicity) HIS3 amino acid starvation). Surprisingly, level CUP1-lacZ HIS3-lacZ activation approximates transcription. These promoter activities increased similarly from either episomal or genomic constructs. Our results emphasize universality mechanism which yeast promoters. Moreover, a comparison absolute for different suggests in relatively constant activation, while normal induction vary considerably. data argue may play uniquely dominant role regulation certain